A3 - Resolving the mode of action of the CRISPR-Cas class I signature nuclease Cas3 inside cells with high spatiotemporal resolution

Project-related Publications

[P1] K.J.A. Martens, B. Turkowyd, U. Endesfelder*
“Raw data to results: a hands-on introduction and overview of computational analysis for single-molecule localization microscopy”
Front. Bioinform. 2021, 1. DOI: 10.3389/fbinf.2021.817254.

[P2] B. Turkowyd, S. Schreiber, J. Wörtz, E.S. Segal, M. Mevarech, I.G. Duggin, A. Marchfelder*, U. Endesfelder*
“Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii”
Front. Microbiol. 2020 11, 583010. DOI: 10.3389/fmicb.2020.583010.

[P3] B. Turkowyd, H. Müller-Esparza, V. Climenti, N. Steube, U. Endesfelder*, L. Randau*
“Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance”
Meth. Enzymol. 2019, 616, 133–171. DOI: 10.1016/bs.mie.2018.11.001.

[P4] I. Vojnovic, J. Winkelmeier, U. Endesfelder*
“Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology”
Biochem. Soc. Trans. 2019, 47, 1041–1065. DOI: 10.1042/BST20180399.

[P5] U. Endesfelder*
“From single bacterial cell imaging towards in vivo single-molecule biochemistry studies”
Essays Biochem. 2019, 63, 187–196. DOI: 10.1042/EBC20190002.

[P6] A. Balinovic, D. Albrecht, U. Endesfelder*
“Spectrally red-shifted fluorescent fiducial markers for optimal drift correction in localization microscopy”
J. Phys. D: Appl. Phys. 2019, 52, 204002. DOI: 10.1088/1361-6463/ab0862.

[P7] D. Virant, B. Traenkle, J. Maier, P.D. Kaiser, M. Bodenhöfer, C. Schmees, I. Vojnovic, B. Pisak-Lukáts, U. Endesfelder*, U. Rothbauer*
“A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging”
Nat. Commun. 2018, 9, 930. DOI: 10.1038/s41467-018-03191-2.

[P8] D. Virant, B. Turkowyd, A. Balinovic, U. Endesfelder*
“Combining Primed Photocon-version and UV-Photoactivation for Aberration-Free, Live-Cell Compliant Multi-Color Single-Molecule Localization Microscopy Imaging”
Int. J. Mol. Sci. 2017, 18, 1524. DOI: 10.3390/ijms18071524.

[P9] B. Turkowyd, A. Balinovic, D. Virant, H.G.G. Carnero, F. Caldana, M. Endesfelder, D. Bourgeois, U. Endesfelder*
“A general mechanism of photoconversion of green‐to‐red fluorescent proteins based on blue and infrared light reduces phototoxicity in live‐cell single‐molecule imaging”
Angew. Chem. Int. Ed. 2017, 56, 11634–11639. DOI: 10.1002/anie.201702870.

[P10] U. Endesfelder*, S. Malkusch, F. Fricke, M. Heilemann*
“A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment”
Histochem. Cell Biol. 2014, 141, 629–638. DOI: 10.1007/s00418-014-1192-3.